Generator

Part:BBa_K1021030:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)

PtrpC+hph


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for fungal expression and therefore does not contain a bacterial RBS. The part was constructed from BBa_K1021027 and BBa_K1021002.

Source

PtrpC from A. nidulans and hph from E. coli were both taken from the vector pUCATPH

References